MICROFLUIDIC DEVICES
IPTeL Tracking ID: MS-CE-2018-068
The invention relates to microfluidic devices for high dynamic range quantification of nucleic acids, proteins, single cells and molecules. The imperative applications of this invention include drug response monitoring for therapeutics, studying reaction kinetics, viral load quantification for prognosis, quantifying effect of enzyme concentration on reactions etc.
BACKGROUND
Currently, detection and quantification of molecules are carried out in microfluidic or lab on chip platforms which includes recombinase polymerase amplification (RPA), nucleic acid sequence based amplification (NASBA), loop-mediated isothermal amplification (LAMP), strand displacement amplification (SDA), helicase dependent amplification (HDA) etc. The existing technologies for absolute quantification of molecules have narrow dynamic range or require sample pre-processing that include multiple serial dilution steps which may lead to errors
TECHNOLOGY
The invention provides a method for using modular microfluidic device for highly accurate and automated measurement of cells and biomolecules (DNA/RNA sequences, protein quantification) with minimum pre-processing steps.
Key features of the invention:
- Integrated programmable serial dilution mechanism
- Enables both on-chip as well as off-chip amplification and quantification
- Provides option for single cell analysis
- Operates in both droplet-mode and steam-mode
- Eliminates need of syringe pump
- Device can operate in low reaction volume