IPTeL Tracking ID: IDR-CeN-2017-045
Researchers from IISc have designed a method and device for enhanced poration in biological cells for intracellular delivery of molecules. This method does not require any label for detection and also it is tunable and high throughput for intracellular delivery of molecules upto 100 nm to biological cells.
BACKGROUND
Cell membranes have barriers to the transport of molecules from extracellular to intracellular space of cell. Various methods are exploited for delivering molecules to cells by forming pores which includes molecular motors, deformability cytometry, acoustics, lasers, electric field etc. However, the existing techniques provide pore size of ~50 nm for higher uptake efficiency.
TECHNOLOGY
The invention provides a method and device for delivering molecules to cells intracellularly using an air-liquid interface induced squeezing wherein ~100 nm size pores can be formed. This method avoids lysis of cells and also it is label-free with high throughput.
COMMERCIALIZATION
An application for patent has been filed in India. We are seeking a commercial partner for licensing and development of this technology.